yvcI [2019-11-21 16:19:12]

RNA pyrophosphohydrolase, converts primary transcripts to 5’-monophospate RNA

Locus: BSU_34780

Isoelectric point: 4.99

Molecular weight: 18.07 kDa

Protein length: 158 aa

Gene length: 477 bp

Function: initiation of RNA degradation

Product: RNA pyrophosphohydrolase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides
• Information processing → RNA synthesis and degradation → RNases → RNA pyrophosphohydrolase
• Information processing → Targets of second messengers → Targets of (p)ppGpp

Gene

Coordinates: 3,572,413 3,572,889

The protein

Protein family

• Nudix hydrolase (according to UniProt)

Domains

• Nudix hydrolase domain (aa 3-130) (according to UniProt)

Structure

• 3FK9 (the protein from B. halodurans, 59% identity/ 82% similarity)

Expression and Regulation

Operons

• Genes: nahA-yvcJ-glmR-whiA-crh-yvcN
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, in CcpA regulon

  Regulation

  • constitutive expression at both protein and RNA levels PubMed

  Additional information

  • A ncRNA is predicted between 'yvcI' and 'trxB' PubMed

Biological materials

Mutant

• MGNA-B642 (yvcI::erm), available at the NBRP B. subtilis, Japan
• BKE34780 (ΔnahA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTAACCTTCGTCCTGTC, downstream forward: _UP4_TAGAAAGACAAGTCAGGGGG
• BKK34780 (ΔnahA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTAACCTTCGTCCTGTC, downstream forward: _UP4_TAGAAAGACAAGTCAGGGGG

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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